| Week 1 | San Francisco 49ers vs. Pittsburgh Steelers | Sunday, September 10th, 2023 at 1:00pm ET |
| Week 2 | San Francisco 49ers vs. Los Angeles Rams | Sunday, September 17th, 2023 at 4:05pm ET |
| Week 3 | New York Giants vs. San Francisco 49ers | Thursday, September 21st, 2023 at 8:15pm ET |
| Week 4 | Arizona Cardinals vs. San Francisco 49ers | Sunday, October 1st, 2023 at 4:25pm ET |
| Week 5 | Dallas Cowboys vs. San Francisco 49ers | Sunday, October 8th, 2023 at 8:20pm ET |
| Week 6 | San Francisco 49ers vs. Cleveland Browns | Sunday, October 15th, 2023 at 1:00pm ET |
| Week 7 | San Francisco 49ers vs. Minnesota Vikings | Monday, October 23rd, 2023 at 8:15pm ET |
| Week 8 | Cincinnati Bengals vs. San Francisco 49ers | Sunday, October 29th, 2023 at 4:25pm ET |
| Week 9 | Bye Week | |
| Week 10 | San Francisco 49ers vs. Jacksonville Jaguars | Sunday, November 12th, 2023 at 1:00pm ET |
| Week 11 | Tampa Bay Buccaneers vs. San Francisco 49ers | Sunday, November 19th, 2023 at 4:05pm ET |
| Week 12 | San Francisco 49ers vs. Seattle Seahawks | Thursday, November 23rd, 2023 at 8:20pm ET |
| Week 13 | San Francisco 49ers vs. Philadelphia Eagles | Sunday, December 3rd, 2023 at 4:25pm ET |
| Week 14 | Seattle Seahawks vs. San Francisco 49ers | Sunday, December 10th, 2023 at 4:05pm ET |
| Week 15 | San Francisco 49ers vs. Arizona Cardinals | Sunday, December 17th, 2023 at 4:05pm ET |
| Week 16 | Baltimore Ravens vs. San Francisco 49ers | Monday, December 25th, 2023 at 8:15pm ET |
| Week 17 | San Francisco 49ers vs. Washington Commanders | Sunday, December 31st, 2023 at 1:00pm ET |
| Week 18 | Los Angeles Rams vs. San Francisco 49ers | Sunday, January 7th, 2024 at 4:25pm ET |
